Figure 5. Decreased ROS level by N-acetyl cysteine amide (NACA) or catalase expression suppresses the lethality induced by expression of dACOX1^N250S.

(A) The anti-oxidant NACA suppresses the lethality of da>dACOX1^N250S, but cannot suppress the lethality of dACOX1^T2A (n = 3 crosses, >50 flies were counted for one cross). (B) Lifespan of flies that co-express UAS-catalase (green) or maintained on NACA (red) or switched to normal food after eclosion (black) (n > 100 per each). (C) % of expected adult flies of the indicated genotypes. The dACOX1^WT and dACOX1^N250S were expressed using various drivers, including ubiquitous drivers (whole body) or drivers for neuronal, glial, ring gland (endocrine organ), hemocyte (blood cell), or fat body (adipose and metabolic organ) expression (n > 200 progenies were counted across 3 trials, STAR Methods). Mean ± s.e.m. ***p< 0.001, **p < 0.01, n.s., not significant. Statistical analyses were determined by 2-sided Student’s t-test. (D) Time to climb past 7 cm for the indicated fly genotypes (n > 50 per genotype). Statistical analyses are one-way ANOVA followed by a Tukey post-hoc test. Means ± s.e.m. ***p < 0.001; n.s., not significant. Western blot below indicated that dACOX1^N250S expression is not affected by the expression of catalase. (E) Lifespan of flies of the indicated genotypes (n > 50 per each genotype).