KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-PKM2 (D78A4) | Cell Signaling | Cat #4053 |
| Rabbit monoclonal anti-G6PD (D5D2) | Cell Signaling | Cat #12263 |
| Rabbit monoclonal anti-GFPT1 (EPR4854) | Abcam | Cat #ab236053 |
| Mouse IgG1 monoclonal isotype control (MG1–45) | Abcam | Cat #ab18447 |
| Rabbit IgG monoclonal isotype control (EPR25A) | Abcam | Cat #ab199376 |
| Mouse monoclonal anti-CDX2 (CDX2–88) | Biogenex | Cat #MU392A-UC |
| Goat anti-OCT4 | Santa Cruz | Cat #sc-8628 |
| Rabbit anti-NANOG | ReproCELL | Cat #RCAB0002P-F |
| Goat anti-SOX2 | R&D Systems | Cat #AF2018 |
| Mouse monoclonal anti-YAP (2F12) | Abnova | Cat #H00010413-M01 |
| Mouse monoclonal anti-TFAP2C (6E4/4) | Santa Cruz | Cat #sc-12762 |
| Rabbit anti-TFAP2C | Santa Cruz | Cat #sc-8977 |
| Rabbit anti-p-Ezrin (T567)/Radixin (T564)/Moesin (T558) | Cell Signaling | Cat #3141 |
| Rabbit anti-SBNO1 | Abcam | Cat #ab122789 |
| Rabbit anti-GATA3 | Santa Cruz | Cat #sc-9009 |
| Rabbit anti-PARD6B | Santa Cruz | Cat #sc-67393 |
| Mouse monoclonal anti-O-Linked N-Acetylglucosamine (RL2) | Abcam | Cat #ab2739 |
| Rabbit monoclonal anti-pT37/46–4E-BP1 (236B4) | Cell Signaling | Cat #2855S |
| Rabbit monoclonal anti-pS235/236-RPS6 (D57.2.2E) | Cell Signaling | Cat #4858 |
| Rabbit monoclonal anti-mTOR (7C10) | Cell Signaling | Cat #2983 |
| Rabbit monoclonal anti-Tuberin/TSC2 (D93F12) | Cell Signaling | Cat #4308 |
| Rabbit monoclonal anti-pS473-AKT (Ser473) (D9E) | Cell Signaling | Cat #4060 |
| Rabbit anti-pS127-YAP | Cell Signaling | Cat #4911 |
| Rabbit anti-pS757-ULK1 | Cell Signaling | Cat #6888 |
| Mouse monoclonal anti-active Rac1-GTP | NewEast | Cat #26903 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Lectin from Triticum vulgaris (wheat)–WGA (20μg/ml) | Sigma-Aldrich | Cat # L0636 |
| YZ9 (1μM) | Cayman Chemical | Cat #15352 |
| ST045849 (0.8μM) | TimTec | Cat #ST045849 |
| Tunicamycin (5μM) | Sigma-Aldrich | Cat #T7765 |
| 6-Aminonicotinamide (6-AN) (2μM) | Sigma-Aldrich | Cat #A68203 |
| N-Acetylcysteine (NAC) (1mM) | Sigma-Aldrich | Cat #A7250 |
| Hypotaurine (1mM) | Sigma-Aldrich | Cat #H1384 |
| Shikonin (1μM) | Cayman Chemical | Cat #14751 |
| Azaserine (5μM) | Sigma-Aldrich | Cat #A4142 |
| 6-Diazo-5-oxo-L-norleucine (DON) (20μM) | Sigma-Aldrich | Cat #D2141 |
| 2-Deoxy-D-glucose (1μM) | Sigma-Aldrich | Cat #D8375 |
| Aphidicolin (10μM) | Cayman Chemical | Cat #14007 |
| Cytochalasin D (500nM) | Cayman Chemical | Cat #11330 |
| α-Amanitin (100μg/ml) | Cayman Chemical | Cat #17898 |
| Cycloheximide (10μg/ml) | Cayman Chemical | Cat #14126 |
| Torin2 (10nM) | Cayman Chemical | Cat #14185 |
| INK128 (100nM) | Cayman Chemical | Cat #11811 |
| JTE013 (1μM) | Tocris | Cat #2392 |
| SKI-II (0.8μM) | Echelon | Cat #B-0024 |
| NSC26722 (100μM) | Tocris | Cat #2161 |
| BYL719 (1μM) | Cayman Chemical | Cat #16986 |
| BKM120 (0.5μM) | Cayman Chemical | Cat #11587 |
| GDC0941 (0.5μM) | Cayman Chemical | Cat #11600 |
| TGX221 (0.5μM) | Cayman Chemical | Cat #10007349 |
| BX912 (100nM) | Cayman Chemical | Cat #14708 |
| GSK2334470 (50nM) | Cayman Chemical | Cat #18095 |
| MK2206 (200nM) | Cayman Chemical | Cat #11593 |
| Lometrexol (250–500nM) | Cayman Chemical | Cat #18049 |
| Methotrexate (200nM) | Cayman Chemical | Cat #13960 |
| Mizoribine (1μM) | Cayman Chemical | Cat #23128 |
| AVN-944 (50nM) | Cayman Chemical | Cat #21284 |
| Leflunomide (5μM) | Cayman Chemical | Cat #14860 |
| U-13C6-D-Glucose (200μM) | Cambridge Isotope Laboratories | Cat #CLM-1396-PK |
| U-13C3-Pyruvic Acid Sodium Salt (200μM) | Toronto Research Chemicals | Cat #P998904 |
| U-13C3-L-(+)-Lactic Acid Sodium Salt (5mM) | Toronto Research Chemicals | Cat #L113507 |
| [1', 2', 3', 4', 5'-13C5]-Uridine (200μM) | Omicron | Cat #NUC-034 |
| U-13C6-D-Glucosamine hydrochloride | Omicron | Cat #GLC-091 |
| Pregnant Mare Serum Gonadotropin (PMSG) | ProSpec | Cat #hor-272 |
| Chorionic gonadotropin human (hCG) | Sigma-Aldrich | Cat #CG10 |
| Critical Commercial Assays | ||
| Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit | Thermo Scientific | Cat #C10330 |
| Duolink® In Situ PLA® Probe Anti-Rabbit PLUS | Sigma-Aldrich | Cat #DUO92002 |
| Duolink® In Situ PLA® Probe Anti-Mouse MINUS | Sigma-Aldrich | Cat #DUO92004 |
| Duolink® In Situ Detection Reagents Red | Sigma-Aldrich | Cat #DUO92008 |
| Duolink® In Situ Wash Buffers, Fluorescence | Sigma-Aldrich | Cat #DUO82049 |
| Experimental Models: Cell Lines | ||
| HEK293T | This lab | N/A |
| Mouse: embryo culture | This lab | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6C3F1/J | Jackson Laboratory | Stock No: 100010 |
| Oligonucleotides | ||
| CleanCap EGFP mRNA (5moU) | TriLink | Cat #L-7201 |
| mCherry-mTrim21 mRNA | This paper | N/A |
| Recombinant DNA | ||
| pGEMHE-mCherry-mTrim21 | Addgene | Cat #105522 |
| pCMV6-Yap1 | OriGene | Cat #MR226049 |
| pCMV6-Tead4 (ATT) | OriGene | Cat #MR219506 |
| pCMV6-Tfap2c | OriGene | Cat #MR207174 |
| pCMV6-Flag-Yap1 | This paper | N/A |
| pCMV6-Myc-Tead4 (ATG) | This paper | N/A |
| pQCMV-GFP-Tfap2c | This paper | N/A |
| Software and Algorithms | ||
| Graphpad Prism 7 | GraphPad | http://www.graphpad.com/ |
| TraceFinder 3.3 | Thermo Scientific | N/A |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| MetaboAnalyst | (Xia and Wishart, 2011) | https://www.metaboanalyst.ca/ |
| Other | ||
| WPI MICRO-ePORE | World Precision Instruments | N/A |