Figure 9.

Dermal fibroblasts from LS patients show delays in cell spreading. (A) Normal and LS cells were resuspended and seeded on fibronectin-coated surfaces. After 30 min, the cells were fixed, stained with rhodamine–phalloidin and imaged. The area of at least 200 cells was computed using ImageJ software, the resulting size-distribution histograms are shown. (B) Examples of rhodamine–phalloidin-stained cells used for area-distribution determination (A) are shown for Normal and both LS patient cells. Arrows point to microspike structures typical of early spreading stages. Some irregularly spread cells are indicated by arrowheads. Scale bar: 20 µm.