Fig. 3. Apcin-induced mitotic slippage depends on APC/C^Cdc20.

a, Fraction of HCT116 cells in mitosis after 24-hour treatment with combinations of proTAME, apcin (0, 6.2, 12.5, 25, 50 μM) and nocodazole. Mitotic fraction was determined using a high-throughput fixed cell assay. The mean of four biological replicates is shown; error bars represent standard deviation. The experiment was not repeated. Statistical significance was calculated using a two-tailed unpaired t test (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001, **** p-value < 0.0001). Exact p-values shown in Supplementary Table 2. b, hTERT-RPE1 H2B-GFP cells were treated with 10 nM control or Cdc20 siRNA for 24 hours, followed by combinations of nocodazole, apcin (0, 6.2, 12.5, 25 μM), and AZ3146 (4 μM) for 24 hours. The fraction of mitotic cells was determined using a high-throughput fixed cell assay. The mean of four biological replicates from two independent experiments is shown; error bars represent standard deviation. Statistical significance was calculated using a two-tailed unpaired t test (* p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001, **** p-value < 0.0001). Exact p-values are shown in Supplementary Table 2.