Fig 2. Cell encapsulation and 3D cultivation of human iPSC-hepatocytes in a core-shell hydrogel fiber.

(A) Human iPSC-hepatocytes singly dissociated and diluted into Matrigel were encapsulated into the core region of the fiber at a density of 1 × 10⁸ cells / mL, using the cell fiber technology. (B) The iPSC-hepatocytes were cultured for 7 days in the ECM-rich 3D microenvironment, and compacted cell aggregates were acquired in the fiber.