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. 2020 Jun 11;15(6):e0231061. doi: 10.1371/journal.pone.0231061

Fig 1. Viral blood feed time courses.

Fig 1

Ae. aegypti were fed human blood spiked with 500 PFU of virus/ μL (approximately 1,000 PFU per 2 μL feed volume). For dual feeds, this titration included 500 PFU of each virus. Post-feed levels of viral RNA were assessed by qPCR and normalized against endogenous mosquito S7 (ribosomal protein S7) RNA levels. Each time point is represented in relation to the mean level at one-hour post feed. (A) Influenza A levels following a single virus feed. (B) Influenza A levels after dual feed with DENV-2. (C) The first 40 hours of DENV-2 decay. (D) The full DENV-2 time course shows viral replication, beginning at approximately 100 hours, that occurs following the initial decay of the inoculum.