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An. stephensi were held at 21° C (RT) or 4°C for 48 hours immediately post-feeding, or frozen immediately after feeding (-80°C). DNA and RNA were isolated from individual mosquitoes and bovine nucleic acid levels were determined with qPCR using primers targeting bovine cytochrome B. Results are shown as percentages relative to the mean of samples frozen immediately after feeding. **—p = 0.024 (n = 5). ***—p < .0001 (n = 7).
