Extended Data Fig. 8. 3D super-resolution reconstructions of immunofluorescence-labeled ChR2-EYFP on dendrites using INSPR and in vitro methods in biplane setup (depth: 2 – 6.2 μm).

(a) x-y overview of the super-resolution volume of immunofluorescence-labeled ChR2-EYFP on dendrites resolved by INSPR, with a depth of 2 μm from the coverslip. (b–d) x-z slices along the white dashed line in (a), reconstructed using INSPR (b), phase retrieval method based on beads on the coverslip with theoretical index mismatch model (PR+IMM, c), and phase retrieval method based on beads on the coverslip (PR, d). (e–j) Zoomed in x-z views of the areas as indicated by the white boxed regions in (b–d). (k–m) x-z slices along the magenta dashed line in (a), reconstructed using INSPR (k), PR+IMM (l), and PR (m). (n–p) Zoomed in x-z views of the areas as indicated by the white boxed regions in (k–m). (q–s) Intensity profiles along the white dashed lines in (n–p), showing the differences in the axial width of the selected contour are 41% and 26% for PR+IMM and PR as compared to INSPR, respectively. The integration width of the x-z slices in (b–p) in the y direction is 200 nm. Norm.: normalized.