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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Lab Invest. 2019 Aug 13;99(12):1822–1834. doi: 10.1038/s41374-019-0296-8

Figure 2. LDVmax and Dmax are increased in aggressive cancer cells and they depend on an intact MT-network.

Figure 2.

LDs were stained with BODIPY and (A) their maximum velocity (LDVmax) was calculated in NHPrE1, LNCaP and PC-3 cells, breast benign epithelial cells (MCF10A), non-metastatic (MCF-7) and metastatic (MDA) cancer cells, and in pancreatic (PANC-1) cancer cells. (B) The maximum displacement (Dmax), defined by the longest vector of displacement from the point of origin in a track, was assessed in NHPrE1, LNCaP and PC-3 cells. (C) The proportion of directional movement was evaluated in benign and PCa cells. (D) Non-directional movement of an LD in a PCa cell as indicated by the non-linear trajectory (black arrow: starting point; red arrow: ending point). (E) Directional movement of an LD in a PCa cell up to for approximately 2/3 of its length (asterisk) as represented by the linear trajectory (black arrow: starting point; red arrow: ending point). (F) Non-directional movement was assessed using a non-quadratic mean square displacement (MSD) plot. (G) Directional movement was assessed using a quadratic MSD. n=50.Values are mean ± SEM. Student’s unpaired t-test. **P<0.01, ***P<0.001.