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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Lab Invest. 2019 Aug 13;99(12):1822–1834. doi: 10.1038/s41374-019-0296-8

Figure 3. PEDF suppresses LDVmax in prostate cancer cells.

Figure 3.

(A) PEDF (50 kD) levels were analyzed by western blot in NHPrE1, LNCaP, and PC-3 cells and normalized by GAPDH (37 kD). NHPrE1, LNCaP, and PC-3 cells were treated with 10 nM purified PEDF protein for 24 h and (B) LDVmax and (C) Dmax were measured and compared to the untreated cells (CTR). (D) The total number of LDs was counted per single cell in NHPrE1, LNCaP and PC-3 cell lines with or without PEDF treatment. n=50. Values are mean ± SEM. Student’s unpaired t-test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.