Figure 7. MeJA did not induce consistent FRET ratio changes of the SNACS SnRK2 activity reporter in guard cells under the imposed conditions.

The SNACS reporter was analyzed in guard cells in leaf epidermises of plants expressing pUBQ10:OST1-HF in the ost1-3 genetic background. The ratio of YPet to Turquoise GL emission was normalized to the average value over the 5 min before treatment. (A) MeJA (20 µM) did not affect the FRET ratio in guard cells. ABA induced ratio increases in the presence of MeJA. After 20 min treatment with 20 µM MeJA, 20 µM ABA was added. (B) Control experiment for A. 0.1% EtOH (solvent control for MeJA) was added.

Figure 7—figure supplement 1. Combined and averages of single stomate imaging data from experimental sets including experiments in Figure 7.

Red lines show the average ratio changes of all stomata traces. Grey lines denote each single stomate ratio change. For visualization of individual stomate time courses see colored traces in Figure 7. Additional data from independent experimental data sets are included in several panels of Figure 4—figure supplement 2, Figure 6—figure supplement 2, Figure 7—figure supplement 1 and Figure 8—figure supplement 1 as indicated by the total number of stomata analyzed in Supplementary Figure legends. (A) n = 18 stomata, including the data shown in Figure 7A. (B) n = 18 stomata, including the data shown in Figure 7B.