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. 2020 May 26;9:e57410. doi: 10.7554/eLife.57410

Key resources table.

Reagent type
(species) or
resource
Designation Source or reference Identifiers Additional
information
Chemical compound, drug SCH772984 selleckchem S7101
Chemical compound, drug U0126 Sigma U120
Chemical compound, drug Epidermal Growth
Factor (EGF)
Sigma E9644
Antibody Anti-phosphoERK1/2 rabbit, polyclonal Cell Signalling Technology Cat# 9102
RRID:AB_330744
(1:1000) dilution
Antibody Anti-ERK1/2 rabbit, polyclonal Cell Signalling Technology Cat# 9101
RRID:AB_331646
(1:1000) dilution
Commercial assay or Kit Lipofectamine-2000 Invitrogen 11668019
Recombinant
DNA Reagent
pCDNA3 EKAR-EV PMID:21976697
Recombinant
DNA Reagent
pTriEx4 Rap1A-FLARE PMID:31444239
Recombinant
DNA Reagent
pTriEx4 Rap1B-FLARE PMID:31444239
Recombinant
DNA Reagent
pcDNA3 Flag-Rap1GAP PMID:23946483
Recombinant
DNA Reagent
pcDNA3 EMBer7.1 PMID:23227961
Cell Line
(Rattus norvegicus)
PC-12 PMID:1065897 RRID:CVCL_0481
Cell Line
(Homo sapiens)
HEK293-T RRID:CVCL_0063
Software, algorithm FIJI RRID:SCR_014294
Software, algorithm MetaFluor RRID:SCR_002285
Software, algorithm GraphPad Prism RRID:SCR_002798