Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Chemical compound, drug | SCH772984 | selleckchem | S7101 | |
Chemical compound, drug | U0126 | Sigma | U120 | |
Chemical compound, drug | Epidermal Growth Factor (EGF) |
Sigma | E9644 | |
Antibody | Anti-phosphoERK1/2 rabbit, polyclonal | Cell Signalling Technology | Cat# 9102 RRID:AB_330744 |
(1:1000) dilution |
Antibody | Anti-ERK1/2 rabbit, polyclonal | Cell Signalling Technology | Cat# 9101 RRID:AB_331646 |
(1:1000) dilution |
Commercial assay or Kit | Lipofectamine-2000 | Invitrogen | 11668019 | |
Recombinant DNA Reagent |
pCDNA3 EKAR-EV | PMID:21976697 | ||
Recombinant DNA Reagent |
pTriEx4 Rap1A-FLARE | PMID:31444239 | ||
Recombinant DNA Reagent |
pTriEx4 Rap1B-FLARE | PMID:31444239 | ||
Recombinant DNA Reagent |
pcDNA3 Flag-Rap1GAP | PMID:23946483 | ||
Recombinant DNA Reagent |
pcDNA3 EMBer7.1 | PMID:23227961 | ||
Cell Line (Rattus norvegicus) |
PC-12 | PMID:1065897 | RRID:CVCL_0481 | |
Cell Line (Homo sapiens) |
HEK293-T | RRID:CVCL_0063 | ||
Software, algorithm | FIJI | RRID:SCR_014294 | ||
Software, algorithm | MetaFluor | RRID:SCR_002285 | ||
Software, algorithm | GraphPad Prism | RRID:SCR_002798 |