Table 2. Parameters obtained from the analysis of axial lsFCS data on NCI-1703 cells.

Receptor	LRP6-mCherry	LRP6-tdTomato	LRP6-tdTomato	xKremen2-mCherry	xKremen2- mCherry, LRP6
Receptor gene insertion	Stable transfection	Transient transfection	CRISPR/Cas9 genome editing	Transient transfection	Transient transfection
Ligand	DKK2-eGFP	DKK1-eGFP	DKK1-eGFP	DKK1-eGFP	DKK1-eGFP
Receptor density (µm‒2) *	32 ± 16	106 ± 52	21 ± 15	44 ± 33	41 ± 30
KD (nM)	0.26 ± 0.04	0.22 ± 0.03	0.10 ± 0.02	10.3 ± 2.1	0.54 ± 0.06
β	0.43 ± 0.02	0.51 ± 0.02	0.26 ± 0.01	0.45 ± 0.02	0.66 ± 0.03
DG (µm2 s‒1) †	0.27 ± 0.08	0.35 ± 0.19	0.27 ± 0.19	0.25 ± 0.15	0.32 ± 0.18
DR (µm2 s‒1) †	0.30 ± 0.07	0.44 ± 0.34	0.39 ± 0.19	0.19 ± 0.08	0.27 ± 0.09
⟨γG⟩‡	1.22 ± 0.17	1.31 ± 0.15	1.47 ± 0.25	1.36 ± 0.22	1.30 ± 0.22
⟨γR⟩‡	1.41 ± 0.19	1.41 ± 0.16	1.66 ± 0.39	1.42 ± 0.29	1.35 ± 0.16

^*Receptor densities were calculated from the amplitudes of the receptor autocorrelation functions, G_R(0), and the observation area (0.20 µm²).

^† Diffusion coefficients of receptor-bound ligands and receptors were calculated from the translational diffusion times by using D = ω₀²/4τ.

^‡ The photobleaching parameters ⟨γ⟩ are given as the median of the distribution from all scans; the errors denote half the width of the second and third quartile of the distribution.