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Diagram of the SS18-SSX fusion sequence targeted by the fusion-specific antibody (E9X9V) (A). Immunoblotting of E9X9V and E5A2C antibodies in whole cell extracts from human fibroblast cells (CRL7250) and SS cell lines (HSSY2, Aska, SYO1, with and without shRNA-mediated knockdown of SS18-SSX expression) showing specific detection of SS18-SSX fusion protein (B) without cross reactivity with wild-type SS18 (C).
