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. 2020 May 4;165(7):1679–1682. doi: 10.1007/s00705-020-04642-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A. dsRNA profile of Phytophthora cactorum isolate BirchT_KT09 visualized in 1% agarose after 90-min electrophoresis at 120 V. PcRV1 is indicated by an arrow, while the other dsRNA elements present in the same host isolate will be reported elsewhere. The amount of O’GeneRuler Express DNA Ladder (Thermo Scientific) was 0.5 µg. According to the manufacturer, the 1.5-kb band corresponds to 100 ng of DNA per 0.5 ug of ladder. B. Schematic representation of the genomic organization of PcRV1. The open reading frames (ORFs) and the untranslated regions (UTRs) are indicated by open bars and single lines, respectively. The underlined sequence shows a putative slippage site (shifty heptamer), and the putative H-type pseudoknots are shown 37 nt to the right and 5 nt to the left. The first AUG of ORF2 is at nt position 3738. C.. Sequence comparison of the eight conserved motifs [1] in the RdRps of PcRV1 and other representative viruses of the family Totiviridae. Colors indicate aa similarity. Black, 100% similar; dark grey, 80–100% similar; light grey, 60–80% similar; white, less than 60% similar. Virus name abbreviations are the same as in the dendrogram (Fig. 2)