Table 2.
List of primary antibodies
| Antigen | Species/antibody type | Supplier | Catalogue number | Dilution/incubation time and temperature |
|---|---|---|---|---|
| Antibodies used on paraffin sections | ||||
| CD44 | Rat/IgG2b monoclonal | BD Pharmingen | 550,538 | 1:50/1 h, RT |
| CD3 | Rabbit/IgG monoclonal | ZytomedSystems | M3070 | 1:100/16 h, 4 °C |
| MPO | Rabbit/Polyclonal | Thermo Scientific | Rb 373 A | 1:500/16 h, 4 °C |
| CD45R | Rat/IgG2a monoclonal | eBioscience | 14–0452 | 1:100/2 h, RT |
| TNC | Rabbit/Polyclonal | Faissner lab | 1:10.000/1 h, RT | |
| Antibodies used on croystat sections | ||||
| F4/80 | Rat/IgG2b monoclonal | Abcam | Ab6640 | 1:100/1 h, RT |
| CD3e | Armenian hamster/IgG | eBioscience | 16–0031 | 1:100/1 h, RT |
| CD4 | Rat/IgG2a monoclonal | eBioscience | 14–0042-81 | 1:200/1 h, RT |
| CD8a | Rat/IgG2a monoclonal | eBioscience | 16–0081-81 | 1:200/1 h, RT |
| CD11b | Rat/IgG2b monoclonal | eBioscience | 14–0112 | 1:200/1 h, RT |
| CD11c | Armenian hamster/IgG | eBioscience | 14–0114 | 1:100/1 h, RT |
| CD206 | Rat/IgG2a monoclonal | BioRad | MCA2235T | 1:400/1 h, RT |
RT room temperature