Table 2.

Summary of effects of IL-1β and TNFα with or without TGF-β1 on expression of Col I, MMP-1 and αSMA in M-ES-healthy, M-ES-endo, and Ectopic-ES.

			M-ES-healthy		M-ES-endo		Ectopic-ES
			IL-1β (pg /mL) :1–10	TNFα (pg/mL) :10–1000	IL-1β (pg/mL) :1–10	TNFα (pg/mL) :10–1000	IL-1β (pg/mL) :1–100	TNFα (pg/mL) :10–1000
Col 1	mRNA	TGF-β1(−)	N.S.	N.S.	N.S.	N.S.	↑: 1 ↓: 100	↑: 10 ↓: 1000
		TGF-β1(+)	N.S.	N.S.	↑	↑	↓: 10–100	↓: 100–1000
	protein	TGF-β1(−)	N.S.	N.S.	N.S.	N.S.	↓: 100	↓: 1000
		TGF-β1(+)	N.S.	N.S.	N.S.	N.S.	↓: 100	↓: 1000
MMP1	mRNA	TGF-β1(−)	N.S.	N.S.	N.S.	N.S.	↑: 10–100	↑: 100–1000
		TGF-β1(+)	N.S.	N.S.	N.S.	N.S.	N.S.	N.S.
αSMA	mRNA	TGF-β1(−)	N.S.	N.S.	N.S.	N.S.	↓: 10–100	↓: 100–1000
		TGF-β1(+)	N.S.	N.S.	N.S.	N.S.	↓: 10–100	↓: 100–1000
	protein	TGF-β1(−)	N.S.	N.S.	N.S.	N.S.	↓: 10–100	↓: 100–1000
		TGF-β1(+)	N.S.	N.S.	N.S.	N.S.	↓: 10–100	↓: 100–1000

Col I: collagen type I.

MMP-1: matrix metalloproteinase-1.

αSMA: alpha smooth muscle actin.

M-ES-healthy: menstrual endometrial stromal cells of healthy fertile women.

M-ES-endo: menstrual endometrial stromal cells of patients with endometriosis.

Ectopic-ES: endometriotic stromal cells.

N.S.: not significant.

According to the results of the present cell proliferation assays, effects of the highest dose of IL-1β (100 pg/mL) on cell migration, collagen gel contraction in M-ES-healthy and M-ES-endo were excluded for further analyses, due to markedly reduced cell viability.