Figure 1.

Deletion of YJL175W mitigates GFP-op-triggered growth defects. (A) Dubious ORFs of whose deletions showed positive interactions with GFP-op overlapping other ORFs. Gray and blue arrows show dubious ORFs, and the verified ORFs overlapped with them, respectively. (B) Genetic interaction scores of indicated mutants with GFP-op. For each mutant, the scores from two independent experiments are shown. Data were obtained from Kintaka et al.¹³. Genetic interaction score was calculated from colony size differences among control strains. NA: not analyzed. (C,D) Max growth rates (orange bars) and max GFP fluorescence levels (green boxes) of wild type (WT) and indicated mutants grown in the synthetic medium (−Leu/Ura). (C) The max growth rate and max GFP fluorescence p-values. (D) The max GFP fluorescence p-values. (E) Growth curves of WT and the YJL175W deletion mutant with empty vector in synthetic medium (−Leu/Ura). (F) Growth curves and GFP fluorescence of WT and the YJL175W deletion mutant upon GFP-op in synthetic medium (−Leu/Ura).