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. 2020 Jun 11;10:9478. doi: 10.1038/s41598-020-66423-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Study of the adhesion of 3T3 fibroblasts on PTFE and on POx surfaces after 24 h incubation. (a) Density of adherent cells on selected samples (Ref - raw PTFE, PTFE + 30 s - PTFE after 30 s of air plasma treatment, PMEOx – without plasma post-treatment, PMEOx + air – plasma post-treatment in air, PMEOx + Ar – plasma post-treatment in argon) 1 day after preparation. The box represents the median values and interquartile ranges of the number of adherent cells per mm², the whiskers represent minimal and maximal values. **p < 0.01, ***p < 0.001, ****p < 0.0001, compared to ref (b) Density of adherent cells on selected samples after 30 days of aging. *p < 0.05, ***p < 0.001, ****p < 0.0001, compared to ref or PTFE + 30 s, (c) Representative fluorescent images of adherent cells stained by green FDA. Scale bar = 50 μm.