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. 2020 Jun 11;10:9483. doi: 10.1038/s41598-020-66421-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of IS on the number of crossings (a), rearings (b), and groomings (c) in the open field test; n = 44. (a) Data are presented as the mean ± SD due to passed normality test (alpha=0.05). Ordinary ANOVA test has been used to compare the data. Anova – 0.048 / F (DFn, DFd) = F (2, 42) = 3.225; Student t-test: *CON vs 200 IS P = 0.0181. (b) Data are presented as a median (full range) due to unmeet normal distribution condition (alpha = 0.05). Data were analyzed using Kruskal-Wallis test. Kruskal-Wallis test – 0.0175 / Kruskal-Wallis statistic 8.092; Mann-Whitney test: *CON vs 200 IS P = 0.048. (c) Data are presented as a median (full range) due to unmeet normal distribution condition (alpha = 0.05). Data were analyzed using Kruskal-Wallis test. Kruskal-Wallis test – 0.0232 / Kruskal-Wallis statistic 7.529; Mann-Whitney test: *CON vs 200 IS P = 0.0371; *100 IS vs 200 IS P = 0.0103. CON – control group; 100 IS – group receiving IS in the dose of 100 mg/kg b.w./day; 200 IS – group receiving IS in the dose of 200 mg/kg b.w./day.