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. 2020 Jun 11;10:9516. doi: 10.1038/s41598-020-66218-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of relative metabolite concentrations in the head, thorax, and abdomen for the NHD (A), GHD (B) and GLU (C) neurodegenerative models. Each point represents the normalized quantity of a metabolite in a single fly. The NHD, GHD or GLU Drosophila are shown in red dots, the UAS/+ (UAS-Httex1p-Q93 (C1) and UAS-Eaat1-IR (C4)) and Gal4/+ (elav-Gal4 (C2) and Eaat1-Gal4 (C3)) controls in blue and black dots, respectively. The horizontal bars indicate the mean metabolite quantity for each fly genotype. Stars above the graphs indicate p values of Tukey-Kramer tests when NHD, GHD or GLU flies were statistically different from the Gal4 (black stars) and UAS (blue stars) controls. ****p < 0.0001, ***p < 0.001, **p < 0.01 and *p < 0.05.