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. 2020 Jun 11;10:9456. doi: 10.1038/s41598-020-66228-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry of the marker of DNA double-strand breaks gamma-H2AX (phosphorylated histone H2AX) in U-2 OS cells grown for 1, 3 and 7 days on bare Ti-6Al-4V samples (Ti-6Al-4V), on samples coated with as-synthesized silicalite-1 films (SF-RT) or with silicalite-1 films heated up to 500 °C (SF-500), and on the control microscopic glass coverslips (GS). The box plot bold black central line shows the median, and its outer edges represent the 1^st and 3^rd quartile. *Indicates a significant difference from GS, and # shows the difference from SF-RT with p ≤ 0.05.