Fig. 2. Dose, host age, and sex influence MNV disease severity in neonatal mice.
a Groups of 3-day-old BALB/c pups were infected with 108 (n = 21, blue), 107 (n = 10, black), 106 (n = 10, purple), or 105 (n = 11, pink) TCID50 units of MNV-1 by oral gavage and monitored for fecal consistency by palpating their abdomens. b The proportion of mice scoring a 3 or 4 at any time point over the 5 days course of infection is presented as the incidence of diarrhea. c, d Data stratified by age from groups of 3- and 4-day-old BALB/c pups (n = 21 for 3-day old (red); n = 35 for 4-day old (blue)) infected with 108 TCID50 units of MNV-1 by oral gavage and monitored for fecal inconsistency. Data presented as fecal scores over time (c) and incidence (d). e, f Data stratified by sex (n = 8 for female (pink); n = 13 for male (blue)) from groups of 3-day-old BALB/c pups infected with 108 TCID50 units of MNV-1 by oral gavage and monitored for fecal inconsistency. Data presented as fecal scores over time (e) and incidence (f). Error bars denote standard errors of mean in all figures. P values were determined using two-way ANOVA with corrections for multiple comparisons. Source data are provided as a Source data file.