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. 2020 Jun 11;11:2968. doi: 10.1038/s41467-020-16798-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Three-day-old BALB/c pups (n = 13) were infected with 10⁸ TCID₅₀ units of MNV-1. At 2 dpi, viral titers were determined in the indicated segments of the intestinal tract and spleen by plaque assay. b Three-day-old BALB/c pups were infected with 2.5 × 10⁴ pfu of neutral red-labeled MNV-1 by oral gavage At 0.5 dpi (n = 5, white), 1 dpi (n = 8, gray), and 2 dpi (n = 8, black), plaque assays were used to measure newly synthesized virus. Error bars denote standard errors of mean in all figures. Source data are provided as a Source data file.