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. 2020 Jun 11;11:2953. doi: 10.1038/s41467-020-16768-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Relative luciferase activity in HEK-293T cells after transfection for 24 h with each of the selected ISGs and infection with VSV-EBOV GP virus (black bars) or VSV-G (gray bars) at an MOI of 0.5. IFITM3 served as a positive control for inhibition of virus entry. Data are presented as means ± SD, and are representative of three independent experiments. (*) indicates a statistically significant difference (p-values of two-tailed Student’s t-tests; **p < 0.01, ***p < 0.001) for VSV-EBOV GP compared with the negative control; (#) indicates a statistically significant difference (p-values of two-tailed Student’s t-tests; ^###p < 0.001) for VSV-G virus compared with the negative control. b Relative luciferase activity in HEK-293T VP30/L cells after transfection for 48 h with vectors encoding the remaining minireplicon components, each of the 10 selected ISGs, and an internal Renilla luciferase control vector. Data are presented as means ± SD, and are representative of three independent experiments. (*) indicates a statistically significant difference (p-values of two-tailed Student’s t-tests; *p < 0.05, **p < 0.01) from the control. c, d Representative western blot showing expression of EBOV VP40, GP, and NP in VLPs and cell lysate from transfected HEK-293T cells (c). The ISG BST2/tetherin, a known inhibitor of VP40-driven VLP formation, was overexpressed with VP40 alone as a positive control. Data are representative of five independent experiments. Quantification analysis of the viral proteins (VP40, GP, and NP) in VLPs following ISG overexpression compared with the GFP control was performed using ImageJ software (d). The ratio of the band intensity in the VLP to that in the cell lysate is indicated as a percentage. Data are presented as the mean ± SD (n = 5). (*) indicates a statistically significant difference (p-values of two-tailed Student’s t-tests; *p < 0.05, **p < 0.01, ****p < 0.0001) from each of the GFP controls. Source data are provided as a Source Data file.