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. 2020 Jun 11;11:2953. doi: 10.1038/s41467-020-16768-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Interaction between NP and CCDC92 in HEK-293T cells transfected with the indicated combination of expression vectors. Cell lysates were immunoprecipitated with anti-FLAG antibody followed by immunoblotting. Data are representative of three independent experiments. IP, immunoprecipitation. WCE, whole-cell extract. b EBOV NP (magenta) and/or CCDC92 (green) in Huh7.0 VP30 cells infected with EBOV∆VP30 24 h prior to transfection with CCDC92 expression vector were visualized with specific antibodies and analyzed by confocal microscopy. The enlarged images corresponding to the boxed areas are shown next to the original image. Data are representative of two independent experiments. Scale bars, 20 μm. c, d Mapping of the NP–CCDC92 binding regions. HEK-293T cells were transfected with expression vectors for c FLAG-CCDC92 and full-length NP (WT) or its deletion mutants lacking the indicated amino acid residues or d NP WT and FLAG-CCDC92 WT or its deletion mutants lacking the indicated amino acid residues. Cell lysates were immunoprecipitated with anti-FLAG antibody and then immunoblotted with the indicated antibodies. Data are representative of three independent experiments. IP, immunoprecipitation. e HEK-293T VP30 cells transfected for 24 h with expression vectors for the indicated CCDC92 constructs were infected with EBOV∆VP30-luc. Virus-driven Renilla luciferase activity was measured on day 3 post infection. Data are presented as means ± SD, and are representative of three independent experiments. (*) indicates a statistically significant difference (p-values of two-tailed Student’s t-tests; ****p < 0.0001) from the control. Source data are provided as a Source Data file.