TABLE 1.

Antifungal susceptibility of C. albicans: planktonic cells, microplate-based abiotic biofilms, and vaginal epithelium-associated biotic biofilms.

Antifungals	Planktonic MIC (mg/l)			Abiotic biofilm MIC80 (mg/l)			Biotic biofilm MIC80 (mg/l)
	C. albicans strains			C. albicans strains			C. albicans strains
	DAY185	VVC2	VVC4	DAY185	VVC2	VVC4	DAY185	VVC2	VVC4
pH 7.2
Nystatin	4	2	4	32	64	64	32	>32	>32
Clotrimazole	0.5	2	1	>1280	>1280	>1280	1280	1280	>1280
Amphotericin B	1	2	1	1	4	4	2	16	16
pH 4.0
Nystatin	16	32	32	16	64	128	32	32	>32
Clotrimazole	8	8	16	1280	>1280	>1280	>1280	>1280	>1280
Amphotericin B	2	2	4	2	4	16	2	>16	>16

Experiments for antifungal susceptibility testing were repeated on three different occasions in triplicate. Geometric means of planktonic MICs and biofilm MIC80s were calculated and presented in the table. Definitions and detailed methods for planktonic MIC and biofilm MIC80 can be found in the main text.