FIGURE 6.

Study of the possible synergistic effect of ZnO NCs and multiple SW treatments (3 times/day). Four different samples were prepared per assay. Control untreated cells (Ctrl), cells only incubated with 10 μg/mL ZnO NCs for 24 h (ZnO NCs), cells treated with SW (SW) and cells incubated with NCs and treated with SW (ZnO NCs + SW). Cells were treated with SW at: (A) 29.1 MPa, 1000 shots, (B) 39.4 MPa, 500 shots, (C) 39.4 MPa, 1000 shots, (D) 50.3 MPa, 500 shots. Cell viability was recorded 24 h after the SW treatment with the WST-1 proliferation reagent. Data are reported as the cell viability with respect to the control referred as the 100%. Results are shown as mean ± SEM. n = 4. **p < 0.01.