FIGURE 8.

Kinetic of cell death in the ZnO NCs + SW experiment. (A) luminescence, expressed as relative light units (RLU), and (B) fluorescence, expressed as relative fluorescence units (RFU), measurements. KB cells were considered alone (Ctrl), incubated with 10 μg/mL ZnO NCs for 24 h (ZnO NCs), treated with SW alone every 4 h for a total of 3 treatments in a day (SW), or incubated with ZnO NCs and then treated for three times (a treatment every 4 h) with SW (ZnO NCs + SW). Phosphatidylserine exposure (luminescence) and loss of the membrane integrity (fluorescence) were measured before the addition of the reaction mix (pre mix), after the addition but before SW treatment (post mix), after the first (post SW I), second (post SW II) and third (post SW III) SW treatment and 24 h after the last SW irradiation (post 24 h). n = 2.