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. 2020 Jun 5;8:543. doi: 10.3389/fbioe.2020.00543

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Copyright © 2020 Ortiz-Velez, Goodwin, Schaefer and Britton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and secretion of hIL-22 by L. reuteri. (A) Recombinant hIL-22 detected by ELISA in supernatants of recombinant L. reuteri (PRB484) upon induction. L. reuteri expressing pSIP411 was used as control (PRB577). (B) Induction of IL-10 production from Colo205 cells upon treatment with commercial rhIL-22 as compared to bacterially secreted IL-22. Data are mean of n = 3 with error bars representing deviation from the mean; comparisons performed with t-tests (two groups) or analysis of variance (ANOVA) (multiple groups). ***P < 0.001, ****P < 0.0001.