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Bacterially secreted hIL-22 is cleaved. Western blot of induced bacterial supernatant and cell lysates. Cell lysates of PRB484 show a band at ~23 kDa, the expected size for hIL-22 fused to the signal peptide. In comparison, supernatants from induced PRB484 cultures show protein bands much smaller in size, ~4 kDa representing cleaved hIL-22.
