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. 2020 Jun 5;11:1119. doi: 10.3389/fmicb.2020.01119

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Copyright © 2020 Nair and Jain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic of CTD-based mycobacterial detection assay. Illustration here shows the general procedure for the isolation of M. smegmatis cells present in a given biological sample. 1–6 represent the key steps in the assay as detailed in “Methods” section. Step 1 is the coupling of anti-GST antibodies with Dynabeads. CTD-GST protein is then coated on these beads (Step 2). Step 3 involves the incubation of mixed bacterial sample having M. smegmatis cells with the product of step 2. Step 4 involves magnetic separation of the Dynabeads that pulls down M. smegmatis cells. The isolated cells can either be eluted by competition with CTD-GST (Step 5) or be used directly in a PCR (Step 6).