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. 2020 Jun 5;11:1119. doi: 10.3389/fmicb.2020.01119

TABLE 2.

List of oligonucleotides used in the present study.

Oligonucleotide Sequence (5′–3′) Purpose
CTD_GFP_GST_for CCAGAGGATCCGGCGACCAACTACTGACTC Cloning of
CTD_GST_rev GGTGGAATTCTTGCGGCCGCTAGGGCTCCTGG CTD-GST
mnoRTfor TCTGCTTGTTGGTGGACTTG Detection of M. smegmatis
mnoRTrev GTCGAACCCCAAGGACTACA by PCR
LacZ_gn_for GCTGGAGTGACGGCAGTTATCTGGAAG Detection of E. coli
LacZ_gn_rev CAGAAACTGTTACCCGTAGGTAGTCACG by PCR
rpoB_BS_gn_for CCAAGGTACGTGCTACAACCAGCGTC Detection of B. subtilis
rpoB_BS_gn_rev CCTCATAGTTGTAGCCATCCCACGTC by PCR

Underlined sequences represent the restriction enzyme site.