TABLE 2.
Oligonucleotide | Sequence (5′–3′) | Purpose |
CTD_GFP_GST_for | CCAGAGGATCCGGCGACCAACTACTGACTC | Cloning of |
CTD_GST_rev | GGTGGAATTCTTGCGGCCGCTAGGGCTCCTGG | CTD-GST |
mnoRTfor | TCTGCTTGTTGGTGGACTTG | Detection of M. smegmatis |
mnoRTrev | GTCGAACCCCAAGGACTACA | by PCR |
LacZ_gn_for | GCTGGAGTGACGGCAGTTATCTGGAAG | Detection of E. coli |
LacZ_gn_rev | CAGAAACTGTTACCCGTAGGTAGTCACG | by PCR |
rpoB_BS_gn_for | CCAAGGTACGTGCTACAACCAGCGTC | Detection of B. subtilis |
rpoB_BS_gn_rev | CCTCATAGTTGTAGCCATCCCACGTC | by PCR |
Underlined sequences represent the restriction enzyme site.