Table 3.

Antibacterial activity of ethyl acetate and methanol extracts of Curvularia tsudae endophytic in Cynodon dactylon

Crude extract of fungal isolates	Zone of inhibition (mm)a/ test bacterial isolates
	Sab	Efb	Sec	Ecc	Pfc
Ethyl acetate extract of culture filtrate concentrations (%)
100	16.33 ± 0.57**	18.33 ± 0.55	17.33 ± 0.57	17.33 ± 0.57*	18.33 ± 0.57
50	14.33 ± 0.55	15.33 ± 0.57	15.33 ± 0.57	14.33 ± 0.55*	13.33 ± 0.57
25	11.33 ± 0.56**	10.33 ± 0.53	10.33 ± 0.57	10.33 ± 0.57	8.33 ± 0.53**
12	8.33 ± 0.50	5.33 ± 0.50	7.33 ± 0.52**	6.33 ± 0.57	6.33 ± 0.57
Methanol extract of mycelial mat concentrations (%)
100	25.33 ± 0.57**	14.33 ± 0.55	16.33 ± 0.57*	16.33 ± 0.57	18.33 ± 0.57*
50	15.33 ± 0.54	11.33 ± 0.56*	12.33 ± 0.57	13.33 ± 0.57	14.66 ± 0.54
25	11.33 ± 0.56	7.33 ± 0.50	10.33 ± 0.57	11.33 ± 0.56	12.33 ± 0.57
12	9.33 ± 0.51	5.33 ± 0.57**	5.33 ± 0.50	8.33 ± 0.53**	9.33 ± 0.51
Amoxicillin	6.33 ± 0.52	3.33 ± 0.50	5.33 ± 0.57	5.33 ± 0.57	6.33 ± 0.57
Ciprofloxacin	18.33 ± 0.55	16.66 ± 0.57	14.33 ± 0.55	15.33 ± 0.57	9.33 ± 0.57
Chloramphenicol	12.33 ± 0.57	7.33 ± 0.57	9.33 ± 0.51	10.33 ± 0.57	6.33 ± 0.57

Values are mean ± SE of three replicates

^*The antibacterial activity were compared using Duncan’s multiple range test (P = 0.05)

^aValues are mean inhibition zone of three replicates (n = 3) crude extracts were dissolved DMSO (Dimethyl sulfoxide) at 10 mg in 1.5 ml of DMSO. Each well received 6.66 μl/well (20 μgml⁻¹)

^bg positive bacteria; SaStaphylococcus aureus, Ef Enterococcus faecalis

^cg negative bacteria, SeSalmonella enterica,EcEscherichia coli, PfPseudomonas fluorescens