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. 2020 Jun 5;13:103. doi: 10.3389/fnmol.2020.00103

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Copyright © 2020 Si, Li, Ye, Li, Liu, Kuang, Chen and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-335 specifically decreases Erf1 expression, promotes SG formation, and prevents cell apoptosis levels in PC12 cells under OGD stimulation. (A) MiR-335 directly targeted the 3′-UTR of Erf1 mRNA in PC12 cells. The results of the dual-luciferase reporter system assay are shown in figure. (B) Erf1 protein expression was decreased in PC12 cells transfected with miR-335 mimic. (C) Efficiency of Erf1 knockdown in PC12 cells. (D) SG formation was elevated in Erf1-knockdown PC12 cells under OGD stimulation. Cells were stimulated for 6 h under OGD conditions. (E) The apoptosis level was reduced in Erf1-knock PC12 cells under OGD stimulation. Annexin V-FITC/PI double staining assay was used for apoptosis levels of cells detection. X axis: PI; Y axis: Annexin V. control-OGD vs. Erf1-KD-OGD; P < 0.05. *P value < 0.05; ***P value < 0.001; ****P value < 0.0001.