Figure 5.

Tregs elicited by aVD-loaded FM-depots migrate to aorta of ApoE^−/− mice. (A) The expression of Foxp3 (red) was detected by immunofluorescence staining in aortic sinus of ApoE^−/− mice. Nuclei were counterstained with DAPI (blue). Scale bar=200 μm. (B) Foxp3⁺ Treg content in aorta was quantified in a serial of cross sections using an in-house developed software. N = 5–8 mice per group. Two-tailed t-tests were used for statistical significance: **p < 0.01.