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. 2020 Jun 5;11:693. doi: 10.3389/fpls.2020.00693

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Copyright © 2020 Vavrdová, Křenek, Ovečka, Šamajová, Floková, Illešová, Šnaurová, Šamaj and Komis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Demonstration and analysis of MAP65-2 colocalization with cortical microtubules. Hypocotyl epidermal cells of stably transformed seedlings expressing both tagRFP-MAP65-2 and TUA6-GFP as visualized by ACLSM (objective 63×/1.40 NA). (A–C) Overview of TUA6-GFP labeled microtubules (A), tagRFP-MAP65-2 (B), and their overlay (C). (D–F) Magnified views of the boxed area of (A–C) showing again TUA6-GFP tagged microtubules (D), tagRFP-MAP65-2 (E) and the resulting merged image (F). (G,H) Scatterplots showing spatial correlation of green vs. red pixels for the entire field of view shown in C (G) or corresponding to the rectangular ROI (boxed area of C; H). Scale bars = 10 μm, (A–C); 5 μm, (D–F).