Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 11;10:9472. doi: 10.1038/s41598-020-66295-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

VNS significantly reduces the number of infiltrating macrophages in the kidney. (a–e) The number of infiltrating leukocytes is evaluated by flow cytometry analysis. (a) There is no significant difference 24 hours after cisplatin injection, but VNS significantly decreases macrophage infiltration 72 hours later (the number of infiltrating macrophages: 11261.1 ± 865.3 and 8660.8 ± 561.6 cells/ g kidney weight for Cis-sham and Cis-VNS, respectively; n = 4 per group; P = 0.0453). (f) Representative pictures of F4/80 staining of kidney sections. (g) The number of macrophages is counted manually using immunohistochemical staining of F4/80 positive cells. (124.3 ± 6.0 and 82.3 ± 8.8 cells/HPF for Cis-sham and Cis-VNS, respectively; n = 3 or 4; P = 0.0095). Data are expressed as mean ± SEM and analyzed using a Student’s t test. *P < 0.05. VNS, vagus nerve stimulation; Cis, cisplatin; HPF, high power field.