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. 2020 Jun 5;8:475. doi: 10.3389/fbioe.2020.00475

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Copyright © 2020 Zhang, Zhang, Luo, Yan, Niibe, Egusa, Zhang, Xie and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Odontoblastic differentiation of O9-1 cells and induced pluripotent stem cell-derived neural crest-like cells (iNCLCs) in vitro. (A) The morphology of differentiated O9-1 cells and iNCLCs under odontogenic differentiation with or without bone morphogenetic protein 4 (BMP-4) administration. (B) Immunofluorescence was performed to detect the expression of odontogenic markers dentin matrix acidic phosphoprotein 1 (DMP-1) and dentin sialophosphoprotein (DSPP). Nuclei were stained with 4′6-dia-mino-2-phenylindole (DAPI). (C) Alkaline phosphatase (ALP) staining. (D) The expression of Msx-1, Runx-2, Dmp-1, and Dspp was evaluated using real-time reverse transcription PCR (RT-PCR).