Fig. 7.

Immunophenotyping of CD71⁺ erythroid cells in the spleen of offspring from advanced maternal aged (AMA) dams. (a) Gating strategy used to quantify CD71⁺ erythroid cells. Proportion of CD71⁺ erythroid cells (b) in the neonatal spleen at 1 week of age and (c) in the infant spleen at 3 weeks of age (n = 8–19 each) in control and AMA groups. Mid‐lines indicate medians, boxes indicate interquartile ranges and whiskers indicate minimum–maximum range. The P‐values were determined by an unpaired t‐test or a Mann–Whitney U‐test. Significant P‐values are shown in bold type.