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. 2020 Jun 5;8:526. doi: 10.3389/fbioe.2020.00526

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Copyright © 2020 Lin, Nielsen and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intact BGC capturing for natural products discovery. (A) Direct cloning method based on enzyme digestion and ligation, including ligation or Gibson assembly-based cloning of BGCs, such as CATCH. (B) Direct cloning method based on long-amplicon PCR and ligation, such as the DiPaC method. (C) Linear–linear homologous recombination (LLHR) mediated by full RecET in E. coli or transformation-associated recombination (TAR) in yeast for cloning BGCs. (D) Site-direct recombination for cloning BGC in vivo, including ϕBT1 integrase-mediated in vivo site-specific recombination, Cre/loxP plus BAC. (E) Site-direct recombination for cloning BGC in vitro, including iCatch, ϕBT1 integrase-mediated in vitro recombination.