Figure 2.

Immunogenicity of the recombinant P. gingivalis antigens synthesized using the wheat germ cell-free translation system (CellFree Science). (A) SDS-PAGE analysis of the recombinant proteins to confirm protein purity. The numbers exhibited on top of the SDS-PAGE gels were recombinant protein numbers shown in Table 2. Approximately, 50 ng of each recombinant protein was loaded into each lane. (B) Serum IgG responses to the recombinant proteins by dot blot analysis with mixed serum used in the immunoaffinity columns (clear bands, smears, and healthy individuals). Approximately, 50 ng of each protein was blotted on the membrane, and sera were used at a dilution of 1:2,500. The left panel shows a scheme of the proteins spotted on the membrane. The numbers exhibited on the left panel are the recombinant protein numbers shown in Table 2.