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. 2020 Jun 5;11:1029. doi: 10.3389/fimmu.2020.01029

Figure 3.

Figure 3

Decrease of pro-inflammatory CD4+ and CD8+ effector T-cells in brains of aged APP-PS1 transgenic mice. (A) Schematic of flow cytometry analysis of cerebral lymphoid cells derived from APP-PS1 mouse brains. T-cells (TCRβ+) are identified in lymphoid cell population (CD45+, CD11b-) and subdivided into CD4+ T-helper cells and CD8+ cytotoxic T-cells. FMO = fluorescence minus one control. Numbers in gates and quadrants indicate percent cells of parental gate. (B) Baseline numbers of CD4+ and CD8+ T-cells increase during aging. However, no difference is observed between non-tg and APP-PS1 tg animals. (C,D) Flow cytometry analysis of intracellular pro-inflammatory IFNγ- and TNFα-content reveals (C) decreased frequency of IFNγ-producing CD8+ T-cells at 18–20 months of age and (D) reduced frequency of TNFα-secreting CD4+ and CD8+ T-cells starting at 14 months of age in APP-PS1 tg animals. Each symbol represents data from one mouse. 3 m, n = 5 (non-tg), n = 5 (tg); 6 m, n = 7 (non-tg), n = 9 (tg); 8 m, n = 8 (non-tg), n = 7 (tg); 14 m, n = 9 (non-tg), n = 7 (tg); 18-20 m, n = 6 (non-tg), n = 8 (tg). (Data are shown as mean ± SD, *p < 0.05, **p < 0.01, n.s. = non-significant, two-way ANOVA with Bonferroni's multiple comparisons test).