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. 2020 May 15;12(5):327. doi: 10.3390/toxins12050327

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) LC-HRMS chromatogram (system A) of C. tropicalis extract and high resolution full scan mass spectra acquired in positive mode on the apex of the peak at (A1) 6.008 min for 44-methyl gambierone isomer and (A2) at 6.592 min for 44-methyl gambierone. HRMS/MS spectra of [M+H]⁺ (m/z 1039.4931) for (B1) 44-methyl gambierone isomer at 6.008 min and for (B2) 44-methyl gambierone at 6.592 min, resulting from an average of three collision energies (15, 30 and 45 eV). Fragments common to both compounds were marked in red in the mass spectrum and reported in Table 3.