Table 6.
List of MRM transitions (m/z) used in ESI- to detect MTXs, gambierone toxins and gambieric acids on system B (API 4000QTrap).
| Compound | MRM transitions (m/z) | CE (eV) | CXP (eV) | |
|---|---|---|---|---|
| MTX1 | 1689.8 >1689.6 | [M-2H]2−/[M-2H]2− | −40 | −15 |
| 1689.8 > 96.9 | [M-2H]2−/[HOSO3]2− | −125 | −21 | |
| 1126.2 > 1126.2 | [M-3H]3−/[M-3H]3− | −40 | −15 | |
| 1126.2 > 96.9 | [M-3H]3−/[HOSO3]3− | −125 | −21 | |
| MTX2 | 1637.5 > 1637.5 | [M-2H]2−/[M-2H]2− | −40 | −15 |
| 1637.5 > 96.9 | [M-2H]2−/[HOSO3]2− | −125 | −21 | |
| 1091.5 > 1091.5 | [M-3H]3−/[M-3H]3− | −40 | −15 | |
| 1091.5 > 96.9 | [M-3H]3−/[HOSO3]3− | −125 | −21 | |
| MTX4 | 1646.2 > 1646.2 | [M-2H]2−/[M-2H]2− | −40 | −15 |
| 1646.2 > 96.9 | [M-2H]2−/[HOSO3]2− | −125 | −21 | |
| desulfo-MTX1 | 1649.8 > 1649.8 | [M-2H]2−/[M-2H]2− | −40 | −15 |
| 1649.8 > 96.9 | [M-2H]2−/[HOSO3]2− | −125 | −21 | |
| didehydro-demethyl- desulfo-MTX1 |
1641.8 > 1641.8 | [M-2H]2−/[M-2H]2− | −40 | −15 |
| 1641.8 > 96.9 | [M-2H]2−/[HOSO3]2− | −125 | −21 | |
| Gambierone | 1023.5 > 1023.5 | [M-H]−/[M-H]− | −40 | −15 |
| 1023.5 > 96.9 | [M-H]−/[HOSO3]− | −125 | −21 | |
| 44-methylgambierone | 1037.6 > 1037.6 | [M-H]−/[M-H]− | −40 | −15 |
| 1037.6 > 96.9 | [M-H]−/[HOSO3]− | −125 | −21 | |
| Gambieroxide | 1193.6 > 1193.6 | [M-H]−/[M-H]− | −20 | −15 |
| 1193.6 > 96.9 | [M-H]−/[HOSO3] − | −125 | −21 | |
| Gambieric acid A | 1055.1 > 1055.1 | [M-H]−/[M-H]− | −20 | −15 |
| 1055.1 > 1037.1 | [M-H]−/[M-H-H2O] − | −40 | −15 | |
| Gambieric acid B | 1069.1 > 1069.1 | [M-H]−/[M-H]− | −20 | −15 |
| 1069.1 > 1051.1 | [M-H]−/[M-H-H2O] − | −40 | −15 | |
| Gambieric acid C | 1183.7 > 1183.7 | [M-H]−/[M-H]− | −20 | −15 |
| 1183.7 > 1165.7 | [M-H]−/[M-H-H2O] − | −40 | −15 | |
| Gambieric acid D | 1197.7 > 1197.7 | [M-H]−/[M-H]− | −20 | −15 |
| 1197.7 > 1179.7 | [M-H]−/[M-H-H2O] − | −40 | −15 | |