Table 1.
Primer sequences designed to detect and identify Chinook salmon bafinivirus (CSBV). Primers used to clone the target sequence and the primers used to check the two large deletions in the polyprotein Iab gene are also included.
| Name | Sequence (5′ to 3′) |
|---|---|
| CSBV for | CATCGACCCAACTAAAGATGCAGC |
| CSBV fint | CAATTGAAACAGTTGAAGGAGG |
| CSBV rev | GTTCAACGTGAGTTCTTGTCAC |
| CSBV rint | CGAGTGTTTGACCGTACTTGTC |
| CSBV qFor | TACGATCCCACCAAAGTACTC |
| CSBV qRev | GTGACATGATCTTCAGTAATGG |
| CSBV probe | (6FAM)AGTTCAAGTTGTTGATTGGGCAGGTA(MGB) |
| CSBV cloning for | CGGTCAACCACACCCAACTTAC |
| CSBV cloning rev | GGAGTCTACATTAATTGGGATG |
| CSBVdel1 for | CCCTGGAGATACTAAATTGG |
| CSBVdel1 rev | TGGTTTTGGTGTTGTCC |
| CSBVdel2 for | GATGTCCCTTACACCTGG |
| CSBVdel2 rev | CACTGTTCCAGTCTACTGTAGC |