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. 2020 Apr 27;12(5):281. doi: 10.3390/toxins12050281

Figure 2.

Figure 2

Initial viability of N2a cells observed in 96-well microplates after 26 h growth in 5% FBS (full line) and 10% FBS (dotted line) culture medium, at different cell seeding density. Six distinct MTT incubation times were also tested: 15 min (blue); 25 min (green); 35 min (orange); 45 min (black); 55 min (pink); 65 min (red). Data represent the mean ± SD of one microplate (N2a cells at 536 P), each point tested in six wells. Mean CVs were <3%. Absorbance values were measured at 570 nm via the MTT assay.