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Initial viability of N2a cells as assessed in RCV controls (cell layer ≈ 100,000 cell/well obtained after 26 h of growth).
| Variability | Experimental Plates | Absorbance Raw Data (1) | DMSO Control (2) | RCV Control (1)–(2) | CVs (%) |
|---|---|---|---|---|---|
| Inter-assay * | 3 | 1.170 ± 0.050 | 0.043 ± 0.0001 | 1.127 ± 0.050 | 4.4 |
* Data represent the mean ± SD of one microplate in three independent experiments (N2a cells at 662, 663 and 666 P).
