Table 2.
Dose-response curve parameters of cell-based assay (CBA)-N2a when detecting VGSC activators or inhibitors.
| Parameters | P-CTX3C ** | PbTx3 ** | STX ** | dc-STX ** |
|---|---|---|---|---|
| Top absorbance | 1.149 ± 0.028 | 1.182 ± 0.060 | 0.917 ± 0.017 | 0.990 ± 0.038 |
| Bottom absorbance | −0.017 ± 0.028 | 0.057 ± 0.013 | 0.149 ± 0.073 | 0.130 ± 0.049 |
| Hillslope | −1.802 ± 0.074 | −2.052 ± 0.102 | 1.545 ± 0.127 | 1.290 ± 0.126 |
| EC80 * (fg/µL) | 0.787 ± 0.155 | 2950 ± 537 | ND | ND |
| EC50 * (fg/µL) | 1.700 ± 0.354 | 5800 ± 926 | 2982 ± 523 | 15851 ± 3050 |
| EC20 * (fg/µL) | ND | ND | 1195 ± 123 | 5456 ± 1434 |
* Data represent the mean ± SD of one microplate in three independent experiments (N2a cells at 662, 663 and 666 P). ** OV+ conditions: 100/10 and 270/27 µM final concentrations for VGSC activators and inhibitors, respectively. ND = not determined.