Table 4.

Variabilities of EC₈₀ and EC₅₀ for two VGSC activators (P-CTX3C and PbTx3) using the revisited CBA-N2a as assessed under non-destructive O/V treatment conditions.

Variability	O/V Treatment	P-CTX3C		PbTx3
		EC80 (fg/µL)	EC50 (fg/µL)	EC80 (fg/µL)	EC50 (fg/µL)
Inter-assay *	100/10 µM	0.787 ± 0.155	1.700 ± 0.354	2950 ± 538	5800 ± 929
Intra-assay **	85.7/8.57 µM	0.964 ± 0.055	2.004 ± 0.190	3230 ± 270	6241 ± 388
Inter-assay ***	85.7/8.57 µM	0.825 ± 0.236	1.730 ± 0.414	3015 ± 347	5946 ± 788

* Data represent the mean ± SD of one microplate in three independent experiments (N2a cells at 662, 663 and 666 P). ** Data represent the mean ± SD of three microplates in one experiment (N2a cells at 810 P). *** Data represent the mean ± SD of one microplate in three independent experiments (N2a cells at 795, 797 and 798 P).